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I find myself coming back to these calculations over and over again. Some may find this useful too…

Droplet Volume Calculator

Convert measured droplet diameter under the microscope directly into volume.

Droplet Diameter:

μm mm

Volume:

0 pL (picoliters)

0 nL (nanoliters)

Droplet Yield & Frequency Calculator

Calculate total droplet yield and generation rate (Hz) based on droplet diameter and dispersed phase flow rate.

Droplet Diameter:

μm mm

Total Aqueous Flow Rate:

µL/min µL/hr

Run Time Duration:

Minutes Hours

Total Droplets Generated:

0

Scientific: 0

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Generation Rate: 0 Hz (droplets/sec)

Poisson Droplet Loading Calculator

Calculate the probability of single-cell encapsulation based on your cell concentration and droplet volume.

Cell Concentration (cells/mL):

Droplet Volume:

pL nL

Lambda (λ): 0 cells/drop

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Empty (k=0): 0%

Single Cell (k=1): 0%

Multiple Cells (k≥2): 0%

Double Poisson Co-Encapsulation Calculator

Calculate the probability of perfectly pairing exactly one Cell A and one Cell B, plus all triplet+ states.

Cell A Concentration (cells/mL):

Cell B Concentration (cells/mL):

Droplet Volume:

pL nL

Lambda A (λ_A): 0 cells/drop

Lambda B (λ_B): 0 cells/drop

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Empty Droplet (0 cells): 0%

Exactly 1 Cell A (Only): 0%

Exactly 1 Cell B (Only): 0%

Perfect Pairing (1A + 1B): 0%

Doublet Cell A (2A + 0B): 0%

Doublet Cell B (0A + 2B): 0%

All Other Combos (Triplets+): 0%